Saux et al. (2004)利用28S rDNA的证据将离猛蚁属(Apomyrma)也划归为该亚科。Boudinot(2015)又将其移出钝猛蚁亚科,归入离猛蚁亚科(Apomyrminae)。
分类特征为:
Dentiform setae present on clypeus (all known taxa except Apomyrma, Amblyopone mutica, and similar species), labrum (Apomyrma), or both (a few Amblyopone species).
Petiole broadly attached to abdominal segment III, without a distinctly descending posterior face (present in Apomyrma).
The Amblyoponinae (as a tribe of Ponerinae) was first explicitly defined in terms of synapomorphies by Ward (1994). Bolton (2003) raised the group to subfamily status, included the aberrant genus Adetomyrma, and listed their synapomorphies. Saux et al. (2004) redefined the subfamily to include the genus Apomyrma, based on molecular evidence (28S rDNA), and gave the following simple diagnosis:
*Dentiform setae present on clypeus (all known taxa except Apomyrma, Amblyopone mutica, and similar species), labrum (Apomyrma), or both (a few Amblyopone species).
Petiole broadly attached to abdominal segment III, without a distinctly descending posterior face (present in Apomyrma).